ID4 Antibody

Product Discontinued

ID4 Antibody Summary

• Immunogen: Synthetic peptide C-KAVSPVRPSGRKAP, from the N Terminus of the protein sequence according to NP_001537.
• Clonality: Polyclonal
• Host: Goat
• Gene: ID4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:64000
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 25kDa band in human kidney, brain and heart lysates at 1ug/ml, in brain and heart this was accompanied by a 40kDa band. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted size of approx. 19kDda according to NP_001537.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reactivity Notes

Human, Mouse, Rat and Pig.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for ID4 Antibody

• bHLHb27BHLHB27
• Class B basic helix-loop-helix protein 27
• DNA-binding protein inhibitor ID-4
• IDB4
• Inhibitor of DNA binding 4
• inhibitor of DNA binding 4, dominant negative helix-loop-helix protein

Background

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes ina number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo-and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain theHLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA andtranscriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 (PubMed7665172)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ID4
• Entrez:
  • 3400(Human)
• Uniprot:
  • NP_001537.(Human)