ICAM-5 Antibody [Biotin]

Recombinant Human ICAM‑5 protein (Catalog # 1950-M5) was serially diluted 2-fold and captured by Mouse Anti-Human ICAM‑5 Monoclonal Antibody (Catalog # MAB1950) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human ICAM‑5 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1950) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ICAM-5 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ICAM-5
  Ala28-Glu570
  Accession # Q9UMF0
• Specificity: Detects human ICAM-5 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse ICAM-5 is observed and less than 2% cross‑reactivity with recombinant human (rh) ICAM-1, rhICAM-2, and rhICAM-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ICAM5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-5 Antibody [Biotin]

• ICAM5
• ICAM-5
• intercellular adhesion molecule 5
• intercellular adhesion molecule 5, telencephalin
• Telencephalin
• TLCNTelencephalin
• TLNtelencephalin

Background

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38‑55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity. The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4‑5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).

1. Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
2. Yoshihara, Y. et al. (1994) Neuron 12:541.
3. Tain, L. et al. (1997) J. Immunol. 158:928.
4. Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
5. Tain, L. et al. (2000) J. Immunol. 150:243.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-5 Antibody (BAF1950)(1)

Publication using BAF1950

• John R. Pollard, Ofer Eidelman, Gregory P. Mueller, Clifton L. Dalgard, Peter B. Crino, Christopher T. Anderson et al. The TARC/sICAM5 Ratio in Patient Plasma is a Candidate Biomarker for Drug Resistant Epilepsy Frontiers in Neurology 2013-01-03 [PMID: 23293627]

Bioinformatics

• Gene Symbol: ICAM5
• Uniprot:
  • Q9UMF0()