ICAM-5 Antibody [Biotin]

• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ICAM-5 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑5
  Leu31-Arg828 (Pro47Arg)
  Accession # Q60625
• Specificity: Detects mouse ICAM-5 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse (rm) ICAM-2, recombinant rat ICAM-1, and recombinant human (rh) ICAM-1 is observed and less than 2% cross‑reactivity with rhICAM-3, rmICAM-1, and rmVCAM-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ICAM5
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Intercellular adhesion molecule-5 (ICAM‑5), also known as telencephalin, is an integral membrane glycoprotein expressed in neurons of mammalian telencephalons. ICAM‑5 is a member of the immunoglobulin superfamily and shares 38‑55% amino acid homology with other ICAMs. Structurally, ICAM‑5 contains nine Ig domains that included 15 N-glycosylation sites, a single transmembrane region, and C‑terminal cytoplasmic tail (1). As with other members of the ICAM family, ICAM‑5 has been shown to be involved in cellular adhesion. ICAM‑5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) via its first NH2-terminal Ig domain. The ability of ICAM‑5 to bind LFA-1 suggests that ICAM‑5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM‑5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4‑5. Homophilic adhesion activity of ICAM‑5 is regulated by a monomer/tetramer transition. ICAM‑5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM‑5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ICAM5