Recombinant Human hydroxysteroid (17-beta) dehydrogenase 11 His Protein

SDS-Page: Recombinant Human hydroxysteroid (17-beta) dehydrogenase 11 Protein [NBP1-50932] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human hydroxysteroid (17-beta) dehydrogenase 11 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20-285 of Human hydroxysteroid (17-beta) dehydrogenase 11
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MESFVKLFIP KRRKSVTGEI VLITGAGHGI GRLTAYEFAK LKSKLVLWDI NKHGLEETAA KCKGLGAKVH TFVVDCSNRE DIYSSAKKVK AEIGDVSILV NNAGVVYTSD LFATQDPQIE KTFEVNVLAH FWTTKAFLPA MTKNNHGHIV TVASAAGHVS VPFLLAYCSS KFAAVGFHKT LTDELAALQI TGVKTTCLCP NFVNTGFIKN PSTSLGPTLE PEEVVNRLMH GILTEQKMIF IPSSIAFLTT LERILPERFL AVLKQKI

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HSD17B11
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 31.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 5mM DTT, 20% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human hydroxysteroid (17-beta) dehydrogenase 11 His Protein

• 17betaHSD11
• 17betaHSDXI
• 17-beta-hydroxysteroid dehydrogenase 11
• 17-beta-hydroxysteroid dehydrogenase type XI
• 17-beta-hydroxysteroid dehydrogenase XI
• 17bHSD11
• CTCL-associated antigen HD-CL-03
• dehydrogenase/reductase (SDR family) member 8
• DHRS8
• EC 1.1.1
• EC 1.1.1.62
• hydroxysteroid (17-beta) dehydrogenase 11
• member 2,17-beta-HSD 11
• PAN1BRETSDR2,17-BETA-HSD11
• retSDR2
• RetSDR2,17-BETA-HSDXI
• SDR16C2,17BHSD11
• T-cell lymphoma-associated antigen HD-CL-03,17-beta-HSD XI

Background

HSD17B11, also known as DHRS8 (dehydrogenase/reductase SDR family member 8), belongs to the HSD17B family of proteins, which regulate the availability of steroids within various tissues throughout the body. It is widely expressed with highest levels found in retina, pancreas, kidney, liver, lung, adrenal, small intestine, ovary and heart as well as in steroidogenic cells. This protein converts androstan-3-alpha,17-beta-diol (3-alpha-diol) to androsterone, suggesting it may participate in androgen metabolism during steroidogenesis. Recombinant human HSD17B11 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HSD17B11