Human IL-28A/IFN-lambda 2 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-28A/IFN-lambda 2 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IFNL2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-28A/IFN-lambda 2 DuoSet ELISA, 15 Plate

• interleukin-28A
• IFN-lambda 2
• IL28A
• IL-28A
• interferon, lambda 2

Background

Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.

Publications for IL-28A/IFN-lambda 2 (DY1587)(10)

Publications using DY1587

• A Rajamanick, NP Kumar, AN Pandiaraj, N Selvaraj, S Munisankar, RM Renji, V Venkatrama, M Murhekar, JWV Thangaraj, MS Kumar, CPG Kumar, T Bhatnagar, M Ponnaiah, R Sabarinath, V Saravanaku, S Babu Restoration of dendritic cell homeostasis and Type I/Type III interferon levels in convalescent COVID-19 individuals BMC immunology, 2022-10-26;23(1):51. 2022-10-26 [PMID: 36289478] (Human)
• Y Fukuda, T Homma, H Inoue, Y Goto, Y Sato, H Ikeda, C Onitsuka, H Sato, K Akimoto, T Ebato, H Suganuma, T Kawahara, H Mikuni, Y Uchida, S Suzuki, A Tanaka, H Sagara Serum IL-28A/IFN-lambda2 is linked to disease severity of COVID-19 Scientific Reports, 2022-03-31;12(1):5458. 2022-03-31 [PMID: 35361913] (Human)
• GR Kathamuthu, NP Kumar, K Moideen, D Baskaran, S Hissar, BM Shrinivasa, R Sridhar, S Babu Diminished circulating plasma and elevated lymph node culture supernatant levels of IL-10 family cytokines in tuberculous lymphadenitis Cytokine, 2018-06-02;0(0):. 2018-06-02 [PMID: 29871780] (Human)
• Suzuki T, Kawamura K, Li Q, Okamoto S, Tada Y, Tatsumi K, Shimada H, Hiroshima K, Yamaguchi N, Tagawa M Mesenchymal stem cells are efficiently transduced with adenoviruses bearing type 35-derived fibers and the transduced cells with the IL-28A gene produces cytotoxicity to lung carcinoma cells co-cultured. BMC Cancer, 2014-09-25;14(0):713. 2014-09-25 [PMID: 25255777] (Human)
• Dolganiuc A, Kodys K, Marshall C, Saha B, Zhang S, Bala S, Szabo G Type III interferons, IL-28 and IL-29, are increased in chronic HCV infection and induce myeloid dendritic cell-mediated FoxP3+ regulatory T cells. PLoS ONE, 2012-10-10;7(10):e44915. 2012-10-10 [PMID: 23071503] (Human)
• Bierne H, Travier L, Mahlakoiv T, Tailleux L, Subtil A, Lebreton A, Paliwal A, Gicquel B, Staeheli P, Lecuit M, Cossart P Activation of type III interferon genes by pathogenic bacteria in infected epithelial cells and mouse placenta. PLoS ONE, 2012-06-14;7(6):e39080. 2012-06-14 [PMID: 22720036] (Human)
• Hepatitis C virus NS3/4A protease blocks IL-28 production. Eur J Immunol, 2012-09-01;42(9):2374-82. 2012-09-01 [PMID: 22685015] (Human)
• Li Q, Kawamura K, Okamoto S, Fujie H, Numasaki M, Namba M, Nagata M, Shimada H, Kobayashi H, Tagawa M Adenoviruses-mediated transduction of human oesophageal carcinoma cells with the interferon-lambda genes produced anti-tumour effects. Br. J. Cancer, 2011-09-27;105(9):1302-12. 2011-09-27 [PMID: 21952623] (Human)
• Pietila TE, Latvala S, Osterlund P, Julkunen I Inhibition of dynamin-dependent endocytosis interferes with type III IFN expression in bacteria-infected human monocyte-derived DCs. J. Leukoc. Biol., 2010-07-07;88(4):665-74. 2010-07-07 [PMID: 20610797] (Human)
• Stoltz M, Klingstrom J Alpha/beta interferon (IFN-alpha/beta)-independent induction of IFN-lambda1 (interleukin-29) in response to Hantaan virus infection. J. Virol., 2010-06-30;84(18):9140-8. 2010-06-30 [PMID: 20592090] (Human)

FAQs for IL-28A/IFN-lambda 2 (DY1587). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IFNL2