Human IFN-alpha 2/IFNA2 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IFN-alpha 2/IFNA2 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IFN-alpha 2/IFNA2 DuoSet ELISA, 5 Plate

• alpha-2a interferon
• Ifa2
• IFNA
• IFNA2
• IFNA2a
• IFNA2b
• IFNA2c
• IFNAA
• IFNalpha 2
• IFN-alpha 2
• IFN-alpha-2
• IFN-alphaA
• INFA2
• interferon alpha 2b
• interferon alpha A
• interferon alpha-2
• Interferon alpha-A
• interferon, alpha 2
• LeIF A
• MGC125764
• MGC125765

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Publications for IFN-alpha 2 (DY9345-05)(5)

Publications using DY9345-05

• Ghaffarpour, S;Ghazanfari, T;Ardestani, SK;Naghizadeh, MM;Vaez Mahdavi, MR;Salehi, M;Majd, AMM;Rashidi, A;Chenary, MR;Mostafazadeh, A;Rezaei, A;Khodadadi, A;Iranparast, S;Khazaei, HA; Cytokine profiles dynamics in COVID-19 patients: a longitudinal analysis of disease severity and outcomes Scientific reports 2025-04-23 [PMID: 40269030] (Human)
• Crawford, JD;Wang, H;Trejo-Zambrano, D;Cimbro, R;Talbot, CC;Thomas, MA;Curran, AM;Girgis, AA;Schroeder, JT;Fava, A;Goldman, DW;Petri, M;Rosen, A;Antiochos, B;Darrah, E; The XIST lncRNA is a sex-specific reservoir of TLR7 ligands in SLE JCI insight 2023-09-21 [PMID: 37733447] (Human)
• SY Bando, FB Bertonha, SE Vieira, DBL de Oliveir, VN Chalup, EL Durigon, P Palmeira, ACP Curi, CS Faria, L Antonangel, GDP Lauterbach, FA Regalio, RM Cesar, CA Moreira-Fi Blood leukocyte transcriptional modules and differentially expressed genes associated with disease severity and age in COVID-19 patients Scientific Reports, 2023-01-17;13(1):898. 2023-01-17 [PMID: 36650374] (Human)
• J Hong, JH Lee, Z Zhang, Y Wu, M Yang, Y Liao, R de la Rosa, J Scheirer, D Pechacek, N Zhang, Z Xu, T Curiel, X Tan, TH Huang, K Xu PRC2-mediated epigenetic suppression of type I IFN-STAT2 signaling impairs antitumor immunity in luminal breast cancer Cancer Research, 2022-12-16;0(0):. 2022-12-16 [PMID: 36222718] (Human)
• C Wilkinson, J Kyle, M Irimpen, S Stuart, S Mohandass, A Sheperd, KJ Smith, MJ Mullin Improved yield of recombinant human IFN-alpha2b from mammalian cells using heterologous signal peptide approach Protein Expression and Purification, 2022-06-02;198(0):106125. 2022-06-02 [PMID: 35659600] (Human)

FAQs for IFN-alpha 2 (DY9345-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.