HSPC268 Overexpression Lysate

Images

 
Western Blot: C7orf55 Overexpression Lysate (Adult Normal) [NBL1-08562] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C7orf55.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HSPC268 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203326 and based on accession number NM_197964. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromosome 7 open reading frame 55 (C7orf55), nuclear gene encoding mitochondrial protein, mRNA.
Gene
C7ORF55

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HSPC268 Overexpression Lysate

  • C7orf55
  • chromosome 7 open reading frame 55
  • FLJ35699
  • FMC1
  • formation of mitochondrial complexes 1 homolog
  • HSPC268

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-88885
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow
NBP1-82493
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-15524
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-41176
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
NBP1-86182
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-49563
Species: Hu
Applications: WB, IHC, IHC-P
H00728695-B01P
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-89306
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-37327
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
H00001350-P01
Species: Hu
NBP1-92563
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF5329
Species: Hu
Applications: WB, IHC
NBP1-88927
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-25196
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, In vitro, CyTOF-ready
NB600-607
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P
NBP1-85599
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-23795
Species: Hu, Rt
Applications: WB

Publications for HSPC268 Lysate (NBL1-08562) (0)

There are no publications for HSPC268 Lysate (NBL1-08562).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HSPC268 Lysate (NBL1-08562) (0)

There are no reviews for HSPC268 Lysate (NBL1-08562). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HSPC268 Lysate (NBL1-08562) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-08562

Additional HSPC268 Products

Bioinformatics Tool for HSPC268 Lysate (NBL1-08562)

Discover related pathways, diseases and genes to HSPC268 Lysate (NBL1-08562). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on HSPC268

There are no specific blogs for HSPC268, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HSPC268 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol C7ORF55
Novus 100% Guarantee