HSPB8/HSP22 Antibody

Western Blot: HSPB8/HSP22 Antibody [NB100-1123] - Staining of Human Muscle lysate (RIPA buffer, 30 ug total protein per lane). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NB100-1123] - Staining of paraffin embedded Human Tonsil. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: HSPB8/HSP22 Antibody [NB100-1123] - Analysis of H11/HSP22 in Human Muscle lysate using NB100-1123 at 1 ug/mL.

Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NB100-1123] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HSPB8/HSP22 Antibody Summary

• Description: Novus Biologicals Goat HSPB8/HSP22 Antibody (NB100-1123) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-HSPB8/HSP22 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence NELPQDSQEVTCT corresponding to C-Terminus according to NP_055180.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HSPB8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 ug/mL
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1 ug/mL
• Application Notes: WB: Approx. 20-25 kDa band observed in human skeletal muscle lysate (predicted MW of ~22 kDa band according to NP_055180).
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HSPB8/HSP22 Antibody

• Alpha-crystallin C chain
• CMT2L
• CRYAC
• DHMN2
• E2IG1
• E2IG1E2-induced gene 1 protein
• H11
• H11DHMN2
• heat shock 22kDa protein 8
• heat shock 27kDa protein 8
• heat shock protein beta-8
• HMN2
• HMN2A
• HSP22
• HSP22CMT2L
• HSPB8
• Protein kinase H11
• Small stress protein-like protein HSP22

Background

Hsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSPB8/HSP22 Antibody (NB100-1123)(1)

Publication using NB100-1123

• Benndorf R, Sun X, Gilmont RR et al. HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27). J Biol Chem 2001-07-20 [PMID: 11342557]

Bioinformatics

• Gene Symbol: HSPB8
• Entrez:
  • 26353(Human)
  • 62353(Mouse)
• Uniprot:
  • NP_055180(Human)