Hsp25 Antibody [PE]

Product Discontinued

Hsp25 Antibody [PE] Summary

• Immunogen: Recombinant mouse Hsp25.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: Hspb1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100

Reactivity Notes

Recognizes Mouse, Rat, Bovine, Canine, Guinea Pig and Hamster HSP25.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Hsp25 Antibody [PE]

• heat shock protein beta-1
• 27kDa
• heat shock protein 1
• Hsp25

Background

In response to adverse changes in their environment, cells from many organisms increase expression of a class of proteins referred to as heat shock or stress proteins. Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa. Studies on estrogen-induced proteins in breast cancer cells suggest a direct relationship between elevated stress protein expression and neoplasia. Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g., phorbol esters) and calcium ionophores1 and elevated levels of Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock resembles the ecdysteroid and heat shock activation of the drosophila Hsp20 genes. This antibody reacts with both the phosphorylated and the non-phosphorylated forms of Hsp25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Hspb1