![Western Blot: HPSC152 Antibody (3E5) [H00051504-M09] - Analysis of HSPC152 expression in transfected 293T cell line by HSPC152 monoclonal antibody (M09), clone 3E5. Lane 1: HSPC152 transfected lysatE (14.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HPSC152-Antibody-3E5-Western-Blot-H00051504-M09-img0002.jpg "Western Blot: HPSC152 Antibody (3E5) [H00051504-M09] - Analysis of HSPC152 expression in transfected 293T cell line by HSPC152 monoclonal antibody (M09), clone 3E5. Lane 1: HSPC152 transfected lysatE (14.2 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 3E5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | HSPC152 (AAH17172, 1 a.a. ~ 125 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKLLTHNLLSSHVRGVGSRGFPLRLQATEVRICPVEFNPNFVARMIPKVEWSAFLEAADNLRLIQVPKGPVEGYEENEEFLRTMHHLLLEVEVIEGTLQCPESGRMFPISRGIPNMLLSEEETES |
| Specificity | HSPC152 (3E5) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TRMT112 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25851604) |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00051504-M09 | Applications | Species |
|---|---|---|
| Zorbas C, Nicolas E, Wacheul L et al. The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis. Mol Biol Cell 2015-06-01 [PMID: 25851604] (WB, ICC/IF, Human) | WB, ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
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