Hornerin Antibody

Immunocytochemistry/Immunofluorescence: Goat Polyclonal Hornerin Antibody [NB100-93405] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Flow Cytometry: Goat Polyclonal Hornerin Antibody [NB100-93405] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Hornerin Antibody Summary

• Description: Novus Biologicals Goat Hornerin Antibody (NB100-93405) is a polyclonal antibody validated for use in ELISA. Anti-Hornerin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QYATQHGEYDTLNK corresponding to internal region according to NP_001009931.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HRNR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 50 kDa band in human skin lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 282 kDa band according to NP_001009931.1. The 50 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Hornerin Antibody

• hornerin
• intermediate filament-associated protein
• S100A16
• S100a18

Background

Hornerin may play a role in cornification

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hornerin Antibody (NB100-93405)(1)

Publication using NB100-93405

• Wang L, Wang YY, Cao Q et al. Hornerin gene was involved in a case of acute myeloid leukemia transformed from myelodysplastic syndrome with t(1;2)(q21;q37). Leukemia 2006-12-01 [PMID: 17051244]

Bioinformatics

• Gene Symbol: HRNR
• Entrez:
  • 388697(Human)
• Uniprot:
  • Q86YZ3(Human)