HOIP/RNF31 Antibody

Western Blot: HOIP/RNF31 Antibody [NB100-1094] - (0.5ug/ml) staining of JURKAT lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HOIP/RNF31 Antibody Summary

• Immunogen: Peptide with sequence C-EEVPLGQSIPRRRK corresponding to C-Terminus according to NP_060469.4.
• Predicted Species: Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RNF31
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5-1.5 ug/ml
• Application Notes: WB: Approx. 120 kDa band observed in HepG2 lysate (calculated MW of 120 kDa band according to NP_060469.4).
• Theoretical MW: 119 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HOIP/RNF31 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HOIP/RNF31 Antibody

• EC 6.3.2.-
• FLJ10111
• FLJ23501
• HOIP
• MGC19975
• ring finger protein 31
• RNF31
• ZIBRA
• ZIBRAHOIL-1-interacting protein
• Zinc in-between-RING-finger ubiquitin-associated domain protein

Background

This protein contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOIP/RNF31 Antibody (NB100-1094)(4)

Publications using NB100-1094

• Yu K, Phu L, Varfolomeev E et al. Immunoaffinity enrichment coupled to quantitative mass spectrometry reveals ubiquitin-mediated signaling events J. Mol. Biol. 2015-06-05 [PMID: 25861760] (WB, Human)
• Varfolomeev E, Goncharov T, Vucic D. Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-kB Signaling Methods Mol. Biol. 2015-03-04 [PMID: 25736754] (WB, Human)
• Varfolomeev E, Izrael-Tomasevic A, Yu K et al. Ubiquitination profiling identifies sensitivity factors for IAP antagonist treatment. Biochem. J. 2014-11-21 [PMID: 25423073] (Human)
• van der Reijden BA, Erpelinck-Verschueren CA, Lowenberg B, Jansen JH. TRIADs: a new class of proteins with a novel cysteine-rich signature. Protein Sci 1999-07-01 [PMID: 10422847]

Bioinformatics

• Gene Symbol: RNF31
• Entrez:
  • 55072(Human)
• Uniprot:
  • Q96EP0()