![Western Blot: HMGB4 Antibody [NBP1-93780] - Analysis in control (vector only transfected HEK293T lysate) and HMGB4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/HMGB4-Antibody-Western-Blot-NBP1-93780-img0007.jpg "Western Blot: HMGB4 Antibody [NBP1-93780] - Analysis in control (vector only transfected HEK293T lysate) and HMGB4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: PPSSFLLFCQDHYAQLKRENPNWSVVQVAKATGKMWSTATDLEKHPYEQRVALLRAKYFEELELYRKQCNARKKYRMS |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HMGB4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | HMGB4 |
![Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining in human testis and endometrium tissues using anti-HMGB4 antibody. Corresponding HMGB4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HMGB4-Antibody-Immunohistochemistry-Paraffin-NBP1-93780-img0006.jpg "Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining in human testis and endometrium tissues using anti-HMGB4 antibody. Corresponding HMGB4 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferus ducts.](http://images.novusbio.com/fullsize/HMGB4-Antibody-Immunohistochemistry-Paraffin-NBP1-93780-img0002.jpg "Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferus ducts.")
![Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/HMGB4-Antibody-Immunohistochemistry-Paraffin-NBP1-93780-img0005.jpg "Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining of human endometrium shows low expression as expected.")
![Simple Western Casein Kinase 2 alpha Antibody (844720) [Unconjugated]](https://images.novusbio.com/images2/Casein_Kinase_2_alpha_MAB7957_Simple_Western_17150.jpg)
![Western Blot Casein Kinase 2 alpha Antibody (844720) [Unconjugated]](https://images.novusbio.com/images2/Casein_Kinase_2_alpha_MAB7957_Western_Blot_13018.jpg)
![Immunocytochemistry Casein Kinase 2 alpha Antibody (844720) [Unconjugated]](https://images.novusbio.com/images2/Casein_Kinase_2_alpha_MAB7957_Immunocytochemistry_13098.jpg)
![Western Blot RB1 Antibody (607121) [Unconjugated]](https://images.novusbio.com/images2/RB1_MAB6495_Western_Blot_10216.jpg)
![Immunocytochemistry RB1 Antibody (607121) [Unconjugated]](https://images.novusbio.com/images2/RB1_MAB6495_Immunocytochemistry_10464.jpg)
![Simple Western RB1 Antibody (607121) [Unconjugated]](https://images.novusbio.com/images2/RB1_MAB6495_Simple_Western_16629.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")