Recombinant Human HMGB2 Protein

SDS-Page: HMGB2 Recombinant Protein [NBP1-72386]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human HMGB2 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-209 of Human HMGB2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH TGSMGKGDPN KPRGKMSSYA FFVQTCREEH KKKHPDSSVN FAEFSKKCSE RWKTMSAKEK SKFEDMAKSD KARYDREMKN YVPPKGDKKG KKKDPNAPKR PPSAFFLFCS EHRPKIKSEH PGLSIGDTAK KLGEMWSEQS AKDKQPYEQK AAKLKEKYEK DIAAYRAKGK SEAGKKGPGR PTGSKKKNEP EDEEEEEEEE DEDEEEEDED EE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HMGB2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Theoretical MW: 26.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human HMGB2 Protein

• High mobility group protein 2
• high mobility group protein B2
• high-mobility group box 2
• HMG-2
• HMG2high-mobility group (nonhistone chromosomal) protein 2

Background

High mobility group protein B2, also known as HMGB2, is a member of the non-histone chromosomal high-mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. Recombinant human HMGB2 protein, fused to His-tag at N-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HMGB2