HMGB2 Antibody (3D2)

Western Blot: HMGB2 Antibody (3D2) [NBP1-69801] - Analysis of anti-HMGB2 antibody with HeLa NE.

Immunocytochemistry/Immunofluorescence: HMGB2 Antibody (3D2) [NBP1-69801] - Analysis of anti-HMGB2 antibody with heLa cell.

Immunohistochemistry-Paraffin: HMGB2 Antibody (3D2) [NBP1-69801] - Analysis of anti-HMGB2 antibody with human testis at concentration 5 ug/ml.

Immunohistochemistry-Paraffin: HMGB2 Antibody (3D2) [NBP1-69801] - Analysis of anti-HMGB2 antibody with human tonsil at concentration 5 ug/ml.

Immunohistochemistry-Paraffin: HMGB2 Antibody (3D2) [NBP1-69801] - Analysis of anti-HMGB2 antibody with human placenta at concentration 5 ug/ml.

ELISA: HMGB2 Antibody (3D2) [NBP1-69801] - Detection limit for recombinant GST tagged HMGB2 is approximately 0.03 ng/ml as a capture antibody.

Product Discontinued

HMGB2 Antibody (3D2) Summary

• Immunogen: HMGB2 (AAH00903, 1 aa - 196 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMGB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for HMGB2 Antibody (3D2)

• High mobility group protein 2
• high mobility group protein B2
• high-mobility group box 2
• HMG-2
• HMG2high-mobility group (nonhistone chromosomal) protein 2

Background

This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGB2