HLCS Antibody

Images

 
Western Blot: HLCS Antibody [NBP2-16856] - Sample (30 ug of whole cell lysate) A: K562 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: HLCS Antibody [NBP2-16856] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

HLCS Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HCS. The exact sequence is proprietary.
Localization
Cytoplasm; Mitochondrion
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HLCS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HLCS Antibody

  • Biotin apo-protein ligase
  • biotin--protein ligase
  • EC 6.3.4.-
  • HCSholocarboxylase synthetase, EC 6.3.410holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)) ligase)
  • holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase(ATP-hydrolysing)) ligase)
  • holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)] ligase)

Background

Holocarboxylase synthetase (EC 6.3.4.10) covalently links biotin to propionyl-CoA carboxylase (PCCA; MIM 232000), pyruvate carboxylase (PC; MIM 608786), alpha-methylcrotonyl-CoA carboxylase (MCCC1; MIM 609010), and acetyl-CoA carboxylase (ACACA; MIM 200350).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol HLCS
Uniprot