Human HLA DQB1 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Suitable Sample Type
Tissue Homogenates, Cell Lysates and other Biological Fluids
Standard Curve Range
0.312-20 ng/mL
Sensitivity
0.125 ng/mL

Order Details

Human HLA DQB1 ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
0.312-20 ng/mL
Sensitivity
0.125 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
HLA-DQB1

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Human HLA DQB1 ELISA Kit (Colorimetric)

  • DC-1 alpha chain
  • DC-alpha
  • HLA class II histocompatibility antigen, DQ alpha 1 chain-like
  • HLA-DCA
  • MHC class II DQA1

Background

HLA-DQB1 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol HLA-DQB1