HLA DQ1/DQ3 Antibody (HL-37) [DyLight 405]

• Reactivity: Hu, Po
• Applications: WB, Flow, IP, CyTOF-ready
• Clone: HL-37
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 405

HLA DQ1/DQ3 Antibody (HL-37) [DyLight 405] Summary

• Immunogen: Burkitt's lymphoma cell line Raji
• Specificity: The antibody HL-37 reacts with polymorphic determinant on human HLA-DQ1 and HLA-DQ3 molecules (recognized epitope was found on isolated beta chain of DQ1), but does not react with HLA-DQ2. DQ is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DQA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Cross-reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for HLA DQ1/DQ3 Antibody (HL-37) [DyLight 405]

• CELIAC1
• HLA class II histocompatibility antigen, DQ beta 1 chain
• HLA-DQB
• IDDM1
• MHC class II antigen DQB1
• MHC class II antigen HLA-DQ-beta-1
• MHC class II DQ beta chain
• MHC class II HLA-DQ beta glycoprotein

Background

HLA-DQB1 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of analpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system bypresenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells(APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains 6 exons.Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes thetransmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the betachain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 differentmolecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DQA1