Histone H2B, member S [p Ser14] Antibody (C.15200186) - BSA Free

Western Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - HeLa cells were treated with colcemid to block the cell cycle in metaphase and histone extracts of these cells were analysed using the antibody against H2BS14p.

Dot Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - To test the cross reactivity of the antibody against H2BS14p, an analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. This figure shows a high specificity of the antibody for the modification of interest.

ELISA: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - To determine the titer of the antibody, analysis was performed using a serial dilution of the antibody against H2BS14p.The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.

• Reactivity: Hu
• Applications: WB, DB, ELISA
• Clone: C.15200186
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Histone H2B, member S [p Ser14] Antibody (C.15200186) - BSA Free Summary

• Description: Novus Biologicals Mouse Histone H2B, member S [p Ser14] Antibody (C.15200186) - BSA Free (NBP2-62794) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Histone H2B containing the phosphorylated Serine 14 (H2BS14p)
• Modification: p Ser14
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: H2BFS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:2000
  • ELISA 1:100
  • Western Blot 1:200

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Purity: Protein A purified

Alternate Names for Histone H2B, member S [p Ser14] Antibody (C.15200186) - BSA Free

• H2B histone family S member10H2B/s
• H2B histone family, member S
• Histone H2B.s

Background

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2BFS