Histone H2a [ubiquitylated Lys119] Antibody - BSA Free

Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - Analysis was performed on whole cell extracts from HeLa cells (25 ug, lane 1), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H2AK119ub.

Chromatin Immunoprecipitation (ChIP): Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - Assays were performed using human HeLa cells, the antibody against H2AK119ub and optimized PCR primer pairs for qPCR. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ELISA: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - To determine the titer of the antibody, an analysis was performed using a serial dilution of the Diagenode antibody against H2AK119ub. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:23,000.

Dot Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - To test the cross reactivity of the antibody against H2AK119ub, a Dot Blot analysis was performed with peptides containing other histone ubiquitinylations and unmodified sequences. This Figure shows a high specificity of the antibody for the modification of interest.

• Reactivity: Hu
• Applications: WB, DB, ELISA, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Histone H2a [ubiquitylated Lys119] Antibody - BSA Free Summary

• Immunogen: Region of histone H2A containing the ubiquitinylated lysine 119 (H2AK119ub)
• Modification: ubiquitylated Lys119
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H2AC20
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 2 - 5 ug
  • Dot Blot 1:20000
  • ELISA 1:200
  • Western Blot 1:200

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Purity: Affinity purified

Alternate Names for Histone H2a [ubiquitylated Lys119] Antibody - BSA Free

• H2A histone family, member Q
• H2A/q
• H2A1B
• H2AFQH2A
• H2A-GL101
• HIST1H2AB
• histone 2, H2ac
• histone cluster 2, H2ac
• histone H2A type 2-C
• Histone H2A
• Histone H2A/q
• Histone H2A-GL101
• histone IIa
• MGC74460

Background

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2AC20