Recombinant Human Histone Deacetylase 8/HDAC8 His (N-Term) Protein

HPLC: Recombinant Human Histone Deacetylase 8/HDAC8 Protein [NBP1-99513]

Product Discontinued

Recombinant Human Histone Deacetylase 8/HDAC8 His (N-Term) Protein Summary

• Description: Recombinant protein for Human Histone Deacetylase 8/HDAC8
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(Q9BY41)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: HDAC8
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: Activity of rh-HDAC8 was tested using Arg-His-Lys(Ac)-Lys(Ac)-AFC, a fluorogenic, acetylated peptide based on residues 379-382 of p53 as a substrate.
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 25 mM Tris (pH 7.5), 100 mM NaCl, 2.7 mM KCl, 3 mM MgCl2, 10% glycerol.
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Histone Deacetylase 8/HDAC8 His (N-Term) Protein

• EC 3.5.1.98
• HD8
• HDAC8
• HDACL1
• Histone Deacetylase 8
• histone deacetylase-like 1
• RPD3

Background

Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases. HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation. Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor Trichostatin A (TSA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HDAC8