Histone Deacetylase 8/HDAC8 Knockout HeLa Cell Lysate

Images

 

Product Details

Summary
Product Discontinued
View other related Histone Deacetylase 8/HDAC8 Lysates

Order Details


    • Catalog Number
      NBP2-64926
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Histone Deacetylase 8/HDAC8 Knockout HeLa Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,2 bp deletion in exon 1 and 1 bp insertion in exon 1
Gene
HDAC8

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout HeLa Cell

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for Histone Deacetylase 8/HDAC8 Knockout HeLa Cell Lysate

  • EC 3.5.1.98
  • HD8
  • HDAC8
  • HDACL1
  • Histone Deacetylase 8
  • histone deacetylase-like 1
  • RPD3

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-03993
Species: Hu, Mu, Bv, Ca, Pm
Applications: WB
NB100-56340
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P, KO
NB100-1669
Species: Hu
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
NBP2-07996
Species: Hu
NBP2-52486
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC, IF
NB100-1923
Species: Ce
Applications: WB, IHC, IHC-P, IP
NBP2-67451
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB110-58358
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
MAB7940
Species: Hu
Applications: WB, IHC
NBP1-30141
Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
H00008294-P01
Species: Hu
H00008363-P01
Species: Hu
H00008362-P01
Species: Hu
H00008365-M01
Species: Hu
Applications: ELISA, ICC/IF
NBL1-11567
Species: Hu
NB21-2024
Species: Hu, Mu, Rt, Ma
Applications: WB, ChIP, DB, ICC/IF
NLS3775
Species: Hu, Mk
Applications: ICC/IF, IHC, IHC-P
NBP1-85351
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926) (0)

There are no publications for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926) (0)

There are no reviews for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Histone Deacetylase 8/HDAC8 Products

Bioinformatics Tool for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926)

Discover related pathways, diseases and genes to Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926)

Discover more about diseases related to Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926).
 

Pathways for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926)

View related products by pathway.

PTMs for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926)

Learn more about PTMs related to Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926).
 

Research Areas for Histone Deacetylase 8/HDAC8 Lysate (NBP2-64926)

Find related products by research area.

Blogs on Histone Deacetylase 8/HDAC8

There are no specific blogs for Histone Deacetylase 8/HDAC8, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Histone Deacetylase 8/HDAC8 Knockout HeLa Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol HDAC8