![Western Blot: Histone Deacetylase 8/HDAC8 Antibody (SD082-07) [NBP2-67824] - Western blot analysis of Histone Deacetylase 8/HDAC8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for](http://images.novusbio.com/fullsize2/Histone-Deacetylase-8-HDAC8-Antibody-(SD082-07)-Western-Blot-NBP2-67824-img0003.jpg "Western Blot: Histone Deacetylase 8/HDAC8 Antibody (SD082-07) [NBP2-67824] - Western blot analysis of Histone Deacetylase 8/HDAC8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IP |
| Clone | SD082-07 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human Histone Deacetylase 8/HDAC8 aa 70-110. (SwissProt: Q9BY41 Human;SwissProt: Q8VH37 Mouse;SwissProt: B1WC68 Rat) |
| Localization | Nucleus, Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HDAC8 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Knockout Validated: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/HDAC1-Antibody-Knockout-Validated-NB100-56340-img0007.jpg)
![Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/HDAC1-Antibody-Simple-Western-NB100-56340-img0003.jpg)
![Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.](http://images.novusbio.com/images2/HDAC1-Antibody-Western-Blot-NB100-56340-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 preferably binds to nuclear Htt with long Qs. HDAC3 associates exclusively with nuclear inclusion bodies. E3 or N3 cells were fixed and stained with anti-HDAC3 antibodies and visualized by Alexa 546 conjugated secondary antibodies. Arrowheads: inclusion bodies with no HDAC3 signals associated. Arrows: HDAC3 signal-associated inclusion bodies. Bar?=?20 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0111277), licensed under a CC-BY licence.](http://images.novusbio.com/images2/HDAC3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1669-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/images2/HDAC3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1669-img0004.jpg)
![Simple Western: HDAC3 Antibody [NB100-1669] - Lane view shows a specific band for HDAC3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/HDAC3-Antibody-Simple-Western-NB100-1669-img0005.jpg)
![Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.](http://images.novusbio.com/images/HIST1H4F-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBP2-07996-img0001.jpg)
![ELISA: H4/n Antibody (3E7H2) [NBP2-52486] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-ELISA-NBP2-52486-img0008.jpg)
![Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Western-Blot-NBP2-52486-img0009.jpg)
![Flow Cytometry: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Flow-Cytometry-NBP2-52486-img0001.jpg)
![Flow Cytometry: Histone Deacetylase 2/HDAC2 Antibody (SY29-02) [NBP2-67451] - Analysis of Hela cells with HDAC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/Histone-Deacetylase-2-HDAC2-Antibody-(SY29-02)-Flow-Cytometry-NBP2-67451-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (SY29-02) [NBP2-67451] - Staining HDAC2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Histone-Deacetylase-2-HDAC2-Antibody-(SY29-02)-Immunocytochemistry-Immunofluorescence-NBP2-67451-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (SY29-02) [NBP2-67451] - Staining HDAC2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Histone-Deacetylase-2-HDAC2-Antibody-(SY29-02)-Immunocytochemistry-Immunofluorescence-NBP2-67451-img0003.jpg)
![Knockout Validated: YAP1 Antibody [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human YAP1 Polyclonal Antibody (Catalog # NB110-58358) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/YAP1-Antibody-Knockout-Validated-NB110-58358-img0008.jpg)
![Knockout Validated: YAP1 Antibody [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 50 ug/mL of Rabbit Anti-YAP1 Polyclonal Antibody (Catalog # NB110-58358). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/images2/YAP1-Antibody-Knockout-Validated-NB110-58358-img0009.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at 1:200 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear and cytoplasm in trophoblast cells.](http://images.novusbio.com/images2/YAP1-Antibody-Immunohistochemistry-Paraffin-NB110-58358-img0010.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Western-Blot-NBP1-30141-img0012.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Western-Blot-NBP1-30141-img0013.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Western-Blot-NBP1-30141-img0004.jpg)
![SDS-Page: Recombinant Human HIST1H4I Protein [H00008294-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00008294-P01-1.jpg)
![Sandwich ELISA: H4/h Antibody (6D4) [H00008365-M01] - Detection limit for recombinant GST tagged HIST1H4H is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/images2/H4-h-Antibody-(6D4)-Sandwich-ELISA-H00008365-M01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: H4/h Antibody (6D4) [H00008365-M01] - Analysis of monoclonal antibody to HIST1H4H on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/H4-h-Antibody-(6D4)-Immunocytochemistry-Immunofluorescence-H00008365-M01-img0002.jpg)
![Western Blot: H4/j Overexpression Lysate (Adult Normal) [NBL1-11567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H4/j.](http://images.novusbio.com/images/H4-j-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBL1-11567-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in HeLa histone prep.](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0009.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0009.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0010.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining in human fallopian tube and skeletal muscle tissues using anti-CCDC6 antibody. Corresponding CCDC6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0012.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Histone Deacetylase 8/HDAC8 Antibody (NBP2-67824)Discover more about diseases related to Histone Deacetylase 8/HDAC8 Antibody (NBP2-67824).
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| Gene Symbol | HDAC8 |
![Flow Cytometry: Histone Deacetylase 8/HDAC8 Antibody (SD082-07) [NBP2-67824] - Analysis of K562 cells with HDAC8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize2/Histone-Deacetylase-8-HDAC8-Antibody-(SD082-07)-Flow-Cytometry-NBP2-67824-img0001.jpg "Flow Cytometry: Histone Deacetylase 8/HDAC8 Antibody (SD082-07) [NBP2-67824] - Analysis of K562 cells with HDAC8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody")
![Western Blot: Histone Deacetylase 8/HDAC8 Antibody (SD082-07) [NBP2-67824] - Analysis of HDAC8 on K562 cells lysates using anti-HDAC8 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/Histone-Deacetylase-8-HDAC8-Antibody-(SD082-07)-Western-Blot-NBP2-67824-img0002.jpg "Western Blot: Histone Deacetylase 8/HDAC8 Antibody (SD082-07) [NBP2-67824] - Analysis of HDAC8 on K562 cells lysates using anti-HDAC8 antibody at 1/1,000 dilution.")
![Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/images2/HDAC9-Antibody-Western-Blot-NBP2-03993-img0004.jpg)
![Western Blot: Sir2.1 Antibody [NB100-1923] - Western blot using NB100-1923 against His tagged SIR-2.1 full-length protein.](http://images.novusbio.com/images2/Sir2.1-Antibody-Western-Blot-NB100-1923-img0003.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Ovary, carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0003.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Pancreas, carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0004.jpg)
![Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Colon, Carcinoma](http://images.novusbio.com/images2/Histamine-H4R-Antibody-Immunohistochemistry-Paraffin-NLS3775-img0005.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")