Histone Deacetylase 8/HDAC8 Antibody (SD082-07)

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (SD082-07) [NBP2-67824] - Western blot analysis of Histone Deacetylase 8/HDAC8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for

Flow Cytometry: Histone Deacetylase 8/HDAC8 Antibody (SD082-07) [NBP2-67824] - Analysis of K562 cells with HDAC8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Western Blot: Histone Deacetylase 8/HDAC8 Antibody (SD082-07) [NBP2-67824] - Analysis of HDAC8 on K562 cells lysates using anti-HDAC8 antibody at 1/1,000 dilution.

Product Discontinued

Histone Deacetylase 8/HDAC8 Antibody (SD082-07) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Histone Deacetylase 8/HDAC8 aa 61-110 / 377. (SwissProt: Q9BY41 Human; SwissProt: Q8VH37 Mouse; SwissProt: B1WC68 Rat)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HDAC8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Histone Deacetylase 8/HDAC8 Antibody (SD082-07)

• EC 3.5.1.98
• HD8
• HDAC8
• HDACL1
• Histone Deacetylase 8
• histone deacetylase-like 1
• RPD3

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC8