HIST1H3A Antibody

Western Blot: HIST1H3A Antibody [NBP2-16838] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16838] - HIST1H3A antibody detects Histone HIST1H3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIST1H3A protein stained by HIST1H3A antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.

Chromatin Immunoprecipitation (ChIP): HIST1H3A Antibody [NBP2-16838] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-HIST1H3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.

Western Blot: HIST1H3A Antibody [NBP2-16838] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16838] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16838] - HIST1H3A Antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIST1H3A protein stained by HIST1H3A Antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Chromatin Immunoprecipitation: HIST1H3A Antibody [NBP2-16838] - Histone H3 (di-methyl Lys4) antibody immunoprecipitates Histone H3 (di-methyl Lys4) protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of Histone H3 (di-methyl Lys4) antibody The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus. .

Immunoprecipitation: HIST1H3A Antibody [NBP2-16838] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 ug of Histone H3 (di-methyl Lys4) antibody 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HIST1H3A Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide surrounding di-methyl Lys4 of human HIST1H3A. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H3C1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:500-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for HIST1H3A Antibody

• H3 histone family, member A
• H3/A
• H3FAHIST1H3E
• H3FB
• H3FC
• H3FD
• H3FF
• H3FH
• H3FI
• H3FJ
• H3FK
• H3FL
• HIST1H3B
• HIST1H3C
• HIST1H3D
• HIST1H3F
• HIST1H3G
• HIST1H3H
• HIST1H3I
• HIST1H3J
• histone 1, H3a
• histone cluster 1, H3a
• histone H3.1
• Histone H3/a
• Histone H3/b
• Histone H3/c
• Histone H3/d
• Histone H3/f
• Histone H3/h
• Histone H3/i
• Histone H3/j
• Histone H3/k
• Histone H3/l

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H3C1