![Western Blot: HIST1H3A Antibody (S06-4I8) [NBP3-14941] - Western blot detection of HIST1H3A in Jurkat, THP-1, C6, 3T3 cell lysates using HIST1H3A Rabbit mAb (1:1000 diluted). Predicted band size: 15KDa. Observed band size: 17KDa.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-S06-4I8-Western-Blot-NBP3-14941-img0001.jpg "Western Blot: HIST1H3A Antibody (S06-4I8) [NBP3-14941] - Western blot detection of HIST1H3A in Jurkat, THP-1, C6, 3T3 cell lysates using HIST1H3A Rabbit mAb (1:1000 diluted). Predicted band size: 15KDa. Observed band size: 17KDa.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | S06-4I8 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit HIST1H3A Antibody (S06-4I8) (NBP3-14941) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide of human HIST1H3A (Uniprot#: P68431) |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | H3C1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA |
| Preservative | 0.01% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HIST1H3A Antibody (NBP3-14941)Find related products by research area.
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| Gene Symbol | H3C1 |
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (S06-4I8) [NBP3-14941] - Immunohistochemistry of HIST1H3A in paraffin-embedded Human lung cancer tissue using HIST1H3A Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-S06-4I8-Immunohistochemistry-Paraffin-NBP3-14941-img0002.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (S06-4I8) [NBP3-14941] - Immunohistochemistry of HIST1H3A in paraffin-embedded Human lung cancer tissue using HIST1H3A Rabbit mAb at dilution 1/50")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")