Hippocalcin like 4 Overexpression Lysate

Images

 
Western Blot: Hippocalcin like 4 Overexpression Lysate (Adult Normal) [NBL1-11690] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hippocalcin like 4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Hippocalcin like 4 Overexpression Lysate Summary

Description
Transient overexpression lysate of hippocalcin like 4 (HPCAL4) The lysate was created in HEK293T cells, using Plasmid ID RC205253 and based on accession number NM_016257. The protein contains a C-MYC/DDK Tag.
Gene
HPCAL4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Hippocalcin like 4 Overexpression Lysate

  • hippocalcin like 4
  • hippocalcin-like protein 4
  • HLP4DKFZp761G122

Background

The protein encoded by this gene is highly similar to human hippocalcin protein and hippocalcin like-1 protein. It also has similarity to rat neural visinin-like Ca2+-binding protein-type 1 and 2 proteins. This encoded protein may be involved in the calcium-dependent regulation of rhodopsin phosphorylation. The transcript of this gene has multiple polyadenylation sites. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-11690

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Bioinformatics

Gene Symbol HPCAL4