Hexosaminidase A/HEXA Overexpression Lysate

Images

 
Western Blot: HEXA Overexpression Lysate (Adult Normal) [NBL1-11515] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEXA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Hexosaminidase A/HEXA Overexpression Lysate Summary

Description

Hexosaminidase A/HEXA Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC203185

Accession#: NM_000520

Protein Tag: C-MYC/DDK
Gene
HEXA

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
58.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Hexosaminidase A/HEXA Overexpression Lysate

  • beta-hexosaminidase subunit alpha
  • Beta-N-acetylhexosaminidase subunit alpha
  • EC 3.2.1
  • EC 3.2.1.52
  • HEXA
  • hexosaminidase A (alpha polypeptide)
  • Hexosaminidase A
  • Hexosaminidase subunit A
  • MGC99608
  • N-acetyl-beta-glucosaminidase subunit alpha
  • TSD

Background

Hexosaminidase A is the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I). [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Hexosaminidase A/HEXA Lysate (NBL1-11515) (0)

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Bioinformatics

Gene Symbol HEXA