HES7 Overexpression Lysate

Images

 
Western Blot: HES7 Overexpression Lysate (Adult Normal) [NBP2-05999] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HES7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HES7 Overexpression Lysate Summary

Description

HES7 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC219701

Accession#: NM_032580

Protein Tag: C-MYC/DDK
Gene
HES7

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
24.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HES7 Overexpression Lysate

  • bHLH factor Hes7
  • BHLHB37
  • bHLHb37transcription factor HES-7
  • Class B basic helix-loop-helix protein 37
  • hairy and enhancer of split 7 (Drosophila)
  • Hairy and enhancer of split 7
  • hHes7
  • SCDO4

Background

The HES7 gene encodes a member of the hairy and enhancer of split family of bHLH transcription factors. The mouse orthologof this gene is regulated by Notch signaling. The protein functions as a transcriptional repressor, and is implicatedin correct patternin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47791
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
H00008209-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP1-45057
Species: Ca, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF6915
Species: Hu, Mu, Rt
Applications: WB
NBP2-37424
Species: Hu
Applications: ELISA, WB
NBP2-24669
Species: Hu, Mu, Pm
Applications: IHC, IHC-P, WB
423-F8
Species: Hu, Mu
Applications: BA
NBP2-41305
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF3600
Species: Hu
Applications: ICC, WB
H00026508-M07
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-33427
Species: Hu
Applications: ChIP, ICC/IF, WB
NBP2-16818
Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, WB
NBP3-03905
Species: Hu, Mu, Rt
Applications: WB
5026-DL
Species: Mu
Applications: BA
5036-WN
Species: Hu
Applications: BA, BA
NBP1-19613
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, Simple Western, WB

Publications for HES7 Lysate (NBP2-05999) (0)

There are no publications for HES7 Lysate (NBP2-05999).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HES7 Lysate (NBP2-05999) (0)

There are no reviews for HES7 Lysate (NBP2-05999). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HES7 Lysate (NBP2-05999) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HES7 Products

Bioinformatics Tool for HES7 Lysate (NBP2-05999)

Discover related pathways, diseases and genes to HES7 Lysate (NBP2-05999). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HES7 Lysate (NBP2-05999)

Discover more about diseases related to HES7 Lysate (NBP2-05999).
 

Pathways for HES7 Lysate (NBP2-05999)

View related products by pathway.

PTMs for HES7 Lysate (NBP2-05999)

Learn more about PTMs related to HES7 Lysate (NBP2-05999).

Blogs on HES7

There are no specific blogs for HES7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HES7 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol HES7