HES-4 Antibody

Western Blot: HES-4 Antibody [NBP2-42517] - Analysis of HepG2 nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HES-4 Antibody Summary

• Description: Novus Biologicals Goat HES-4 Antibody (NBP2-42517) is a polyclonal antibody validated for use in WB and ELISA. Anti-HES-4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RSAAEHRKVGSRP corresponding to internal region according to NP_001135939.1.
• Specificity: This antibody is expected to recognize isoform 1 (NP_001135939.1) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HES4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 24 kDa band observed in HepG2 nuclear lysates (calculated MW of 26.1 kDa band according to NP_001135939.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HES-4 Antibody

• bHLH factor Hes4
• BHLHB42
• bHLHb42transcription factor HES-4
• Class B basic helix-loop-helix protein 42
• hairy and enhancer of split 4 (Drosophila)
• Hairy and enhancer of split 4
• HES4
• HES-4
• hHES4

Background

Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HES-4 Antibody (NBP2-42517)(1)

Publication using NBP2-42517

• El Yakoubi W, Borday C, Hamdache J et al. Hes4 Controls Proliferative Properties of Neural Stem Cells During Retinal Ontogenesis. Stem Cells 2012-12-01 [PMID: 22969013]

Bioinformatics

• Gene Symbol: HES4
• Entrez:
  • 57801(Human)