HES-4 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

HES-4 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human HES-4
  Val94-Arg221
  Accession # Q9HCC6
• Specificity: Detects human HES-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) HES-1, rhHES-2, rhHES-5, and rhHES-7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HES-4 Antibody [Alexa Fluor® 405]

• bHLH factor Hes4
• BHLHB42
• bHLHb42transcription factor HES-4
• Class B basic helix-loop-helix protein 42
• hairy and enhancer of split 4 (Drosophila)
• Hairy and enhancer of split 4
• HES4
• HES-4
• hHES4

Background

HES-4 is a 221 aa, 24 kDa member of the HES-family of transcriptional repressors. HES-4 contains a species-specific bHLH domain (aa 33‑93), a specific orange DNA-binding domain (aa 109‑150), a 65 aa Proline-rich SH3-binding domain, and a WRPW Groucho/TLE interacting C-terminal motif. HES-4 is a HES-1 paralog likely to maintain stem cell pluripotency. In the region used as immunogen, the amino acid sequence of human and canine HES-4 are 80% identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.