HES-4 Antibody (455237)

Western blot shows lysates of PC-3 human prostate cancer cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human HES-4 Monoclonal Antibody (Catalog # MAB3600) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for HES-4 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

• Reactivity: Hu
• Applications: WB
• Clone: 455237
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HES-4 Antibody (455237) Summary

• Immunogen: E. coli-derived recombinant human HES-4
  Val94-Arg221
  Accession # Q9HCC6
• Specificity: Detects human HES-4 in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HES4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HES-4 Antibody (455237)

• bHLH factor Hes4
• BHLHB42
• bHLHb42transcription factor HES-4
• Class B basic helix-loop-helix protein 42
• hairy and enhancer of split 4 (Drosophila)
• Hairy and enhancer of split 4
• HES4
• HES-4
• hHES4

Background

The Notch pathway maintains embryonic stem cells by up‑regulation of HES and Hey basic helix-loop-helix family members, which repress tissue specific expression. HES-4 is up‑regulated in gastric and colorectal cancers. It shares high homology with HES-1, HES-2, and HES-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HES4
• Uniprot:
  • Q9HCC6()