Hepassocin/FGL1 Antibody

Western Blot: Hepassocin/FGL1 Antibody [NBP1-45216] - Analysis of human Liver lysate (35ug protein in RIPA buffer) using NBP1-45216 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

Hepassocin/FGL1 Antibody Summary

• Immunogen: Peptide with sequence C-ETRVKQQQVKIKQ corresponding to internal region according to NP_004458.3.
• Epitope: internal region
• Specificity: Reported variants represent identical protein: NP_963846.1, NP_963847.1, NP_671736.2, NP_004458.3
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in human liver lysates (calculated MW of 36.4 kDa band according to NP_004458.3).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Hepassocin/FGL1 Antibody

• FGL1
• fibrinogen-like 1
• fibrinogen-like protein 1
• Fibrinogen-like-protein 1
• FREP1
• Hepassocin
• hepatocellular carcinoma-related sequence
• Hepatocyte-derived fibrinogen-related protein 1
• HFREP-1
• HFREP1MGC12455
• HP-041
• LFIRE1
• LFIRE-1
• Liver fibrinogen-related protein 1

Background

Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hepassocin/FGL1 Antibody (NBP1-45216)(1)

Publication using NBP1-45216

• Liu Z, Ukomadu C. Fibrinogen-like protein 1, a hepatocyte derived protein is an acute phase reactant. Biochem Biophys Res Commun 2008-01-25 [PMID: 18039467]

Bioinformatics

• Gene Symbol: FGL1
• Entrez:
  • 2267(Human)
  • 234199(Mouse)
  • 246186(Rat)
• Uniprot:
  • NP_004458.3(Human)