HEMK1 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

HEMK1 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human HEMK1.

  Source: E. coli

  Amino Acid Sequence: VSNPPYVFHQDMEQLAPEIRSYEDPAALDGGEEGMDIITHILALAPRLLKDSGSIFLEVDPRHPELVSSWLQSRPDLYLNLVAVRRDFCG

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: HEMK1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-81019.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for HEMK1 Recombinant Protein Antigen

• EC 2.1.1.-
• FLJ22320
• HEMK homolog 7kb
• HemK methyltransferase family member 1
• HEMK
• m.HsaHemKP
• MTQ1

Background

HEMK1, also known as HemK methyltransferase family member 1, is a 338 amino acid that is 38 kDa; as methyltransferase transferase plays role in the methylation of the GGQ triplet of the mitochondrial translation release factor MTRF1L. This protein is being studied for its involvement in yersinia pseudotuberculosis, infectious disease, neisseria, meningitides, pneumonia, and tuberculosis. The HEMK1 protein has been linked to histidine metabolism and tyrosine metabolism pathways where it interacts with MTRF1, MTRF1L, MAT1A, MAT2A, and AHCY proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HEMK1