HCN4 Antibody (SHG 1E5)

Western Blot: HCN4 Antibody (SHG 1E5) [NB100-74439] - Analysis of 25 ug of rat eye lysate onto an SDS polyacrylamide gel.

Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.

Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, ICC/IF
• Clone: SHG 1E5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

HCN4 Antibody (SHG 1E5) Summary

• Immunogen: Synthetic peptide sequence corresponding to residues S H G S L L L P P A S S P P P P Q V P Q R R G T P P L T P G R L T Q D L K L of HCN4.
• Specificity: HCN4 (SHG 1E5)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: HCN4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:10 - 1:100
  • Western Blot 1:5000
• Application Notes: WB: Detects an approx.132 KDa protein representing HCN4.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for HCN4 Antibody (SHG 1E5)

• HCN4
• hyperpolarization activated cyclic nucleotide-gated cation channel 4
• hyperpolarization activated cyclic nucleotide-gated potassium channel 4
• potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• SSS2

Background

HCN3 is a member of the family of hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. HCN3 has been detected in the brain, but with less expression than other HCN channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HCN4 Antibody (NB100-74439)(2)

Publications using NB100-74439

• Ding Y, Lang D, Yan J et al. A phenotype-based forward genetic screen identifies Dnajb6 as a sick sinus syndrome gene eLife 2022-10-18 [PMID: 36255053] (Immunoprecipitation, Immunoblotting, Human, Rat)

FAQs for HCN4 Antibody (NB100-74439). (Showing 1 - 2 of 2 FAQs).

1. I was looking for HCN4 antiboby that attaches to the extra cellular domain of the channel tested on mouse and human for immunio stoaining..
   • The immunogen for our monoclonal antibody to HCN4, is actually the rat protein. The sequence comes from amino acids 1046 - 1083 of the 1198 amino acid protein, but unfortunately, it is from a cytoplasmic domain. This is the only HCN4 Ab that we have tested for IHC and that has been validated for use with mouse and human.
2. I need an anti human HCN4 antibody for sorting. I was wondering if your product HCN4 antibody (NB110-74735) can react with the extracellular portions of HCN4 and is applicable for FACS?
   • NB110-74735 was raised against a synthetic peptide corresponding to a region that covered both cytoplasmic and extracellular regions of the HCN4 protein. We don't routinely map our antibodies, and we don't know where specifically this antibody binds its target. NB100-74439 may be a better option for you, as the immunogen sequence is provided on our website, but we have not validated it for Flow/FACS.

Bioinformatics

• Gene Symbol: HCN4
• Entrez:
  • 10021(Human)
  • 330953(Mouse)
  • 59266(Rat)
• Uniprot:
  • Q9Y3Q4()