![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-10420249141932.jpg%3Fv=1666704272 "Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against HEK293 (1) and HBO1 (AA: 1-200)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 4E12H12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse HBO1 Antibody (4E12H12) - BSA Free (NBP3-27149) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Purified recombinant fragment of human HBO1 (AA: 1-200) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KAT7 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 70.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HBO1 Antibody (NBP3-27149)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KAT7 |
![ELISA: HBO1 Antibody (4E12H12) [NBP3-27149] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024915839.jpg%3Fv=1666704272 "ELISA: HBO1 Antibody (4E12H12) [NBP3-27149] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)")
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against human HBO1 (AA: 1-200) recombinant protein. (Expected MW is 48.1 kDa)](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024915849.jpg%3Fv=1666704272 "Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mAb against human HBO1 (AA: 1-200) recombinant protein. (Expected MW is 48.1 kDa)")
![Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mouse mAb against MOLT4 (1), COS7 (2), F9 (3), HepG2 (4), PC-2 (5), U251 (6), MCF-7 (7), and NIH/3T3 (8) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27149_mouse-hbo1-mab-4e12h12-bsa-free-1042024920235.jpg%3Fv=1666704272 "Western Blot: HBO1 Antibody (4E12H12) [NBP3-27149] - Analysis using HBO1 mouse mAb against MOLT4 (1), COS7 (2), F9 (3), HepG2 (4), PC-2 (5), U251 (6), MCF-7 (7), and NIH/3T3 (8) cell lysate.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")