H2AFV Overexpression Lysate (Native)

Images

 
Western Blot: H2AFV Overexpression Lysate (Adult Normal) [NBL1-11423] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for H2AFV.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

H2AFV Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200564 and based on accession number NM_012412. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens H2A histone family, member V (H2AFV), transcript variant 1, mRNA.
Gene
H2AFV

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
13.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for H2AFV

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for H2AFV Overexpression Lysate (Native)

  • FLJ26479
  • H2A histone family, member V
  • H2A.F/Z
  • H2AV
  • histone H2A.F/Z
  • histone H2A.V
  • MGC10170
  • MGC10831
  • MGC1947
  • purine-rich binding element protein B

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. Several transcript variants encoding different isoforms, have been identified for this gene. Transcript Variant: This variant (1) encodes the longest protein (isoform 1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol H2AFV