EEF1G Antibody (3F11-1A10) - Azide and BSA Free

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G expression in transfected 293T cell line by EEF1G monoclonal antibody (M01), clone 3F11-1A10.Lane 1: EEF1G transfected lysate(50 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of monoclonal antibody to EEF1G on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - Analysis of EEF1G over-expressed 293 cell line, cotransfected with EEF1G Validated Chimera RNAi ( Cat # H00001937-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EEF1G monoclonal antibody (M01), clone 3F11-1A10 (Cat # H00001937-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in PC-12.

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in HepG2.

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in 293.

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10 Analysis of EEF1G expression in HL-60.

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in Raw 264.7.

Western Blot: EEF1G Antibody (3F11-1A10) [H00001937-M01] - EEF1G monoclonal antibody (M01), clone 3F11-1A10. Analysis of EEF1G expression in NIH/3T3.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 3F11-1A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EEF1G Antibody (3F11-1A10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EEF1G (AAH15813.1, 1 a.a. ~ 437 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHFGQTNRTPEFLRKFPAGKVPAFEGDDGFCVFESNAIAYHVSNEELRGSTPEAAAQVVQWVSFADSDIVPPASTWVFPTLGIMHHDKQATENAKEEVRRILGLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWRKLDPGSEETQTLVREYFSWEGAFQHVGKAFNQGKIFK
• Specificity: EEF1G - eukaryotic translation elongation factor 1 gamma
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EEF1G
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EEF1G Antibody (3F11-1A10) - Azide and BSA Free

• eEF-1B gamma
• EF-1-gamma
• EF1Gelongation factor 1-gamma
• eukaryotic translation elongation factor 1 gamma
• GIG35
• pancreatic tumor-related protein
• PRO1608
• translation elongation factor eEF-1 gamma chain

Background

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EEF1G Antibody (H00001937-M01)(3)

Publications using H00001937-M01

• Blandin G, Marchand S, Charton K et al. A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome. Skelet Muscle 2013-02-15 [PMID: 23414517] (WB, Human)
• Corbi N, Batassa EM, Pisani C et al. The eEF1 gamma Subunit Contacts RNA Polymerase II and Binds Vimentin Promoter Region. PLoS One. 2010-12-31 [PMID: 21217813]
• Prado MA, Casado P, Zuazua-Villar P et al. Phosphorylation of human eukaryotic elongation factor 1Bgamma is regulated by paclitaxel. Proteomics 2007-09-01 [PMID: 17708594] (WB)

Bioinformatics

• Gene Symbol: EEF1G
• Entrez:
  • 1937(Human)
• OMIM:
  • 130593(Human)
• Uniprot:
  • AAH15813(Human)