H1F0 Antibody

Images

 
Western Blot: H1F0 Antibody (N/A) [NBP3-07713] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody.
Immunocytochemistry/ Immunofluorescence: H1F0 Antibody (N/A) [NBP3-07713] - Immunofluorescence staining of PFA-fixed HeLa cells using H1F0 antibody followed by goat anti-rabbit IgG-CF488 (green). Phalloidin is used to ...read more
Immunohistochemistry-Paraffin: H1F0 Antibody (N/A) [NBP3-07713] - Formalin-fixed, paraffin-embedded human Tonsil stained with H1F0 Rabbit Polyclonal Antibody.
Flow Cytometry: H1F0 Antibody (N/A) [NBP3-07713] - Flow Cytometric Analysis of HeLa cells. H1F0 antibody followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Catalog# & Formulation Size Price

H1F0 Antibody Summary

Immunogen
Recombinant full-length human H1F0 protein
Localization
Nuclear
Marker
Pan Nuclear Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H1-0
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Notes

200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08698)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for H1F0 Antibody

  • H1 histone family, member 0
  • H1.0, H1(0), H1-0
  • H10
  • H1FVMGC5241
  • Histone H1'
  • Histone H1(0)
  • histone H1.0

Background

Plasmid: pCMV-H1F0 Full length; Host Cell: 293T

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol H1-0