H1F0 Antibody

Images

 
Western Blot: H1F0 Antibody [NBP2-16772] - HepG2 whole cell extracts and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (NBP2-16772) at a dilution of ...read more
Immunocytochemistry/ Immunofluorescence: H1F0 Antibody [NBP2-16772] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using H1F0 antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments are ...read more
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50
Western Blot: H1F0 Antibody [NBP2-16772] - Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus.
Western Blot: H1F0 Antibody [NBP2-16772] - Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and the membrane was blotted with Histone H1 antibody diluted by 1:5000.
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded rat fore brain. Histone H1.0 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Histone H1 antibody detects H1F0 protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Histone H1 ...read more
Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.06 mg/ml

Order Details

H1F0 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Histone H1.0. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H1F0
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.06 mg/ml
Purity
Immunogen affinity purified

Alternate Names for H1F0 Antibody

  • H1 histone family, member 0
  • H1.0, H1(0), H1-0
  • H10
  • H1FVMGC5241
  • Histone H1'
  • Histone H1(0)
  • histone H1.0

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol H1F0