GTF2H5 Overexpression Lysate

Images

 
Western Blot: GTF2H5 Overexpression Lysate (Adult Normal) [NBL1-11392] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GTF2H5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GTF2H5 Overexpression Lysate Summary

Description

GTF2H5 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC209579

Accession#: NM_207118

Protein Tag: C-MYC/DDK
Gene
GTF2H5

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GTF2H5 Overexpression Lysate

  • bA120J8.2
  • C6orf175trichothiodystrophy
  • FLJ30544
  • General transcription factor IIH polypeptide 5
  • general transcription factor IIH subunit 5
  • general transcription factor IIH, polypeptide 5
  • TFB5 ortholog
  • TFB5chromosome 6 open reading frame 175
  • TFIIH basal transcription factor complex TTDA subunit
  • TFIIH basal transcription factor complex TTD-A subunit
  • TFIIH
  • TTD
  • TTD-A
  • TTDATGF2H5

Background

This gene encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating cellular levels of TFIIH. Mutations in this gene result in trichothiodystrophy, complementation group A. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3296
Species: Hu, Mu, Rt
Applications: WB
NB100-61060
Species: Hu, Mu, V-Vi
Applications: ChIP, IP, WB
NB100-581
Species: Hu, Mu, Rt
Applications: ICC, IHC, IP, WB
NB100-74457
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
H00000902-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NBP3-13174
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
H00002965-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, S-ELISA, WB
NB100-74611
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF2396
Species: Hu
Applications: IHC, Simple Western, WB
NBP3-17971
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, IHC, IHC-P, WB
NBP3-17973
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF3416
Species: Hu
Applications: WB
NBP1-32622
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-477
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-82774
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-06477
Species: Hu
Applications: ICC/IF, IP, WB
NBP3-07348
Species: Hu
Applications: Flow, ICC/IF, PA
H00008878-M01
Species: Bv, Ha, Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-31381
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-37399
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB

Publications for GTF2H5 Lysate (NBL1-11392) (0)

There are no publications for GTF2H5 Lysate (NBL1-11392).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GTF2H5 Lysate (NBL1-11392) (0)

There are no reviews for GTF2H5 Lysate (NBL1-11392). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GTF2H5 Lysate (NBL1-11392) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GTF2H5 Products

Array NBL1-11392

Bioinformatics Tool for GTF2H5 Lysate (NBL1-11392)

Discover related pathways, diseases and genes to GTF2H5 Lysate (NBL1-11392). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GTF2H5 Lysate (NBL1-11392)

Discover more about diseases related to GTF2H5 Lysate (NBL1-11392).
 

Pathways for GTF2H5 Lysate (NBL1-11392)

View related products by pathway.

Research Areas for GTF2H5 Lysate (NBL1-11392)

Find related products by research area.

Blogs on GTF2H5

There are no specific blogs for GTF2H5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GTF2H5 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol GTF2H5