GTF2H5 Overexpression Lysate (Native)

Images

 
Western Blot: GTF2H5 Overexpression Lysate (Adult Normal) [NBL1-11392] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GTF2H5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GTF2H5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209579 and based on accession number NM_207118. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens general transcription factor IIH, polypeptide 5 (GTF2H5), mRNA.
Gene
GTF2H5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GTF2H5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GTF2H5 Overexpression Lysate (Native)

  • bA120J8.2
  • C6orf175trichothiodystrophy
  • FLJ30544
  • General transcription factor IIH polypeptide 5
  • general transcription factor IIH subunit 5
  • general transcription factor IIH, polypeptide 5
  • TFB5 ortholog
  • TFB5chromosome 6 open reading frame 175
  • TFIIH basal transcription factor complex TTDA subunit
  • TFIIH basal transcription factor complex TTD-A subunit
  • TFIIH
  • TTD
  • TTD-A
  • TTDATGF2H5

Background

This gene encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating cellular levels of TFIIH. Mutations in this gene result in trichothiodystrophy, complementation group A. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GTF2H5