Recombinant Mouse GST Epitope Tag Protein

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SDS-Page: Recombinant Mouse GST Epitope Tag Protein [NBP1-30276] - GST Epitope Tag Protein Glutathion S-transferase Mu 1, 28.1 kDa (238aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
1 mg/ml

Order Details

Recombinant Mouse GST Epitope Tag Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-218 of Mouse GST Epitope Tag

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK

Details of Functionality
Specific activity is > 40,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GSTM1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-30276 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Mouse GST Epitope Tag Protein

  • EC 2.5.1.18
  • glutathione S-alkyltransferase
  • glutathione S-aralkyltransferase
  • glutathione S-aryltransferase
  • glutathione S-transferase M1
  • glutathione S-transferase mu 1
  • GST class-mu 1
  • GST HB subunit 4
  • GST1
  • GSTM1-1
  • GSTM1a-1a
  • GSTM1b-1b
  • GTH4
  • GTM1
  • HB subunit 4
  • H-B
  • MGC26563
  • MU
  • MU-1
  • S-(hydroxyalkyl)glutathione lyase

Background

GST Epitope Tag is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GST Epitope Tag protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for GST Epitope Tag Recombinant Protein (NBP1-30276)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol GSTM1