GST Epitope Tag Antibody - BSA Free

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Western Blot: GST Epitope Tag Antibody [NB600-325] - Analysis using the HRP conjugate of NB600-325. Detection of 0.1, 0.3, or 1.0 ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml ...read more

Product Details

Summary
Reactivity ETSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

GST Epitope Tag Antibody - BSA Free Summary

Immunogen
Goats were immunized with Glutathione-S-Transferase (GST) from Schistosoma japonicum. Antibody was isolated by affinity chromatography using GST immobilized on solid support.
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GSTM1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
  • Western Blot 1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Publications
Read Publications using
NB600-325 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GST Epitope Tag Antibody - BSA Free

  • EC 2.5.1.18
  • glutathione S-alkyltransferase
  • glutathione S-aralkyltransferase
  • glutathione S-aryltransferase
  • glutathione S-transferase M1
  • glutathione S-transferase mu 1
  • GST class-mu 1
  • GST HB subunit 4
  • GST1
  • GSTM1-1
  • GSTM1a-1a
  • GSTM1b-1b
  • GTH4
  • GTM1
  • HB subunit 4
  • H-B
  • MGC26563
  • MU
  • MU-1
  • S-(hydroxyalkyl)glutathione lyase

Background

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GST Epitope Tag Antibody (NB600-325)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSTM1