GSK-3 beta [p Ser9] Antibody (SY02-71)

Western Blot: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Analysis of GSK-3 beta [p Ser9] on different lysates with Rabbit anti-GSK-3 beta [p Ser9] antibody at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Staining Phospho-GSK3 beta(Ser 9) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Analysis of paraffin-embedded human pancreas tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.

Western Blot: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Analysis of Phospho-GSK3 beta(Ser 9) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: A549 cell lysate

Western Blot: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Analysis of GSK-3 beta [p Ser9] on Hela cell lysates. Lane 1 : Hela cells, whole cell lysate, 10ug/lane Lane 2 : Hela cells starved for 3 hours, whole cell lysates, 10ug/lane Lane 3/4 : Hela cells starved for 3 hours, then treated with 100nM Calyculin A for 30 minutes, whole cell lysates, 10ug/lane Lane 5 : Hela cells starved for 3 hours and treated with 100nM Calyculin A for 30 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-GSK-3 beta [p Ser9] antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47 kDa Observed band size: 47 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 1 minute 2 seconds

Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Staining Phospho-GSK3 beta(Ser 9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Analysis of paraffin-embedded human breast tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: GSK-3 beta [p Ser9] Antibody (SY02-71) [NBP2-67444] - Analysis of paraffin-embedded human kidney tissue using anti-Phospho-GSK3 beta(Ser 9) antibody. Counter stained with hematoxylin.

Product Discontinued

GSK-3 beta [p Ser9] Antibody (SY02-71) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser9 of Human GSK-3 beta aa 1-50 / 420. (SwissProt: P49841 Human)
• Modification: p Ser9
• Localization: Cytoplasm, Nucleus, Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSK3B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30189238).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GSK-3 beta [p Ser9] Antibody (SY02-71)

• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 beta
• glycogen synthase kinase-3 beta
• GSK3 beta
• GSK-3 beta
• GSK3B
• GSK3beta isoform

Background

Glycogen synthase kinase 3beta (GSK-3 beta) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimer's disease. Additionally, increased GSK-3 beta protein levels are found in Alzheimer's disease brains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GSK-3 beta Antibody (NBP2-67444)(1)

Publication using NBP2-67444

• Wang YH, Liou KT, Tsai KC et al. GSK-3 inhibition through GLP-1R allosteric activation mediates the neurogenesis promoting effect of P7C3 after cerebral ischemic/reperfusional injury in mice. Toxicol. Appl. Pharmacol. 2018-10-15 [PMID: 30189238] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: GSK3B