GSK-3 beta [p Ser9] Antibody (MM0748-19G29)

Product Discontinued

GSK-3 beta [p Ser9] Antibody (MM0748-19G29) Summary

• Immunogen: This antibody was produced from a hybridoma (rat myeloma fused with spleen cells from a rat immunized with Phosphopeptides containing human GSK-3beta S9 site).
• Modification: p Ser9
• Specificity: This antibody recognizes human phosphor-S9 GSK 3beta in direct ELISAs and WBs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: GSK3B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50-200
  • Western Blot 1:1000-2000
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for GSK-3 beta [p Ser9] Antibody (MM0748-19G29)

• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 beta
• glycogen synthase kinase-3 beta
• GSK3 beta
• GSK-3 beta
• GSK3B
• GSK3beta isoform

Background

Glycogen synthase kinase 3beta (GSK-3 beta) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimer's disease. Additionally, increased GSK-3 beta protein levels are found in Alzheimer's disease brains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSK3B
• Uniprot:
  • P49841()