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GSK-3 beta Antibody (272536) [Unconjugated]

Images

 
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat GSK-3 beta Monoclonal Antibody ...read more
GSK-3 beta was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rat Anti-Human/Mouse/Rat GSK-3 beta Monoclonal Antibody (Catalog # MAB2506) at 3 µg/mL for 3 hours at room temperature. ...read more
GSK-3 beta was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rat Anti-Human/Mouse/Rat GSK-3 beta Monoclonal Antibody (Catalog # MAB2506) at 3 µg/mL for 3 hours at room temperature. ...read more
BSSM activates AKT/GSK3 beta pathway, promotes tau hyperphosphorylation and PHF accumulation and increases the expression of active asparagine endopeptidase (AEP) and tau cleavage. (A,B) Western blotting showed higher ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
272536
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

GSK-3 beta Antibody (272536) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841
Specificity
Detects human, mouse, and rat GSK-3 beta  in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha .
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
GSK3B
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB2506 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSK-3 beta Antibody (272536) [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta , inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GSK-3 beta Antibody (MAB2506)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GSK-3 beta Antibody (MAB2506) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSK3B
Uniprot