Recombinant Human GSK-3 alpha Protein

SDS-Page: Recombinant Human GSK-3 alpha Protein [NBP1-99200]

Product Discontinued

Recombinant Human GSK-3 alpha Protein Summary

• Description: Peptide (amino acids 12- 30) surrounding Ser21 of human recombinant protein GSK-3 alpha fused to glutathione S-transferase (GST).
  
  Source:E. coli
  Amino Acid Sequence:(P49840)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab285758)
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GSK3A
• Purity: SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: The recombinant GSK-3 alpha fusion protein may serve as a useful substrate for assaying Akt kinase activity in an in vitro assay.
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from endotoxin free water
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Reconstitute in sterile water.

Alternate Names for Recombinant Human GSK-3 alpha Protein

• DKFZp686D0638
• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 alpha
• glycogen synthase kinase-3 alpha
• GSK3 alpha
• GSK-3 alpha
• GSK3A

Background

GSK-3 is one of the Akt substrates. It can be phosphorylated by Akt at Ser21 of GSK-3alpha or Ser9 of GSK-3Beta . The phosphorylation of GSK negatively regulates its kinase activity. The recombinant GSK-3alpha fusion protein serves as a useful substrate for assaying Akt kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GSK3A