GSK-3 alpha/beta [p Ser9, p Ser21, p Ser9] Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL ...read more
Jurkat human acute T cell leukemia cells, untreated (open histogram, yellow line), or treated with PI3-kinase inhibitors (dark orange filled histogram), were stained with Human/Mouse/Rat Phospho-GSK‑3 alpha / beta ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

GSK-3 alpha/beta [p Ser9, p Ser21, p Ser9] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing GSK-3 beta S9 site
Modification
p Ser9, p Ser9, p Ser21
Specificity
Detects human, mouse, and rat GSK-3 beta when phosphorylated at S9, and human, mouse, and rat GSK‑3 alpha when phosphorylated at S21.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GSK3A
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
AF1590 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSK-3 alpha/beta [p Ser9, p Ser21, p Ser9] Antibody [Unconjugated]

  • DKFZp686D0638
  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 alpha
  • glycogen synthase kinase-3 alpha
  • GSK-3 alpha
  • GSK-3 alpha/beta

Background

GSK-3 is a Ser/Thr kinase first identified as an inactivator of Glycogen Synthase. GSK-3 acts as a multifunctional downstream switch that determines the output of numerous signaling pathways. There are two mammalian GSK-3 isoforms encoded by distinct genes, GSK-3 alpha and GSK-3 beta, which are structurally similar, but functionally non-identical. GSK-3a is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3 alpha and GSK-3 beta share 85% amino acid identity. Dysregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer's disease, bipolar disorder, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GSK-3 alpha/beta Antibody (AF1590)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(5)
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Filter By Species
Human
(1)
Mouse
(1)
Rat
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF1590 Applications Species
Y Tang, KT Kline, XS Zhong, Y Xiao, H Lian, J Peng, X Liu, DW Powell, G Tang, Q Li Chronic colitis upregulates microRNAs suppressing brain-derived neurotrophic factor in the adult heart PLoS ONE, 2021;16(9):e0257280. 2021 [PMID: 34543287] (Western Blot, Rat) Western Blot Rat
Chiu C, Liao C, Shen C, Tang T, Jow G, Wang H, Wu J HYS-32-Induced Microtubule Catastrophes in Rat Astrocytes Involves the PI3K-GSK3beta Signaling Pathway. PLoS ONE, 2015;10(5):e0126217. 2015 [PMID: 25938237] (Western Blot, Rat) Western Blot Rat
Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response. PLoS ONE, 2014;9(1):e86466. 2014 [PMID: 24489729] (Western Blot, Human) Western Blot Human
Kitase Y, Barragan L, Qing H, Kondoh S, Jiang JX, Johnson ML, Bonewald LF Mechanical induction of PGE2 in osteocytes blocks glucocorticoid-induced apoptosis through both the beta-catenin and PKA pathways. J. Bone Miner. Res., 2011;25(12):2381-92. 2011 [PMID: 20578217] (Western Blot, Mouse) Western Blot Mouse
Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation. J. Exp. Med., 2010;207(4):867-80. 2010 [PMID: 20231377] (Western Blot, Rat) Western Blot Rat

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Bioinformatics

Gene Symbol GSK3A