Recombinant Human GRPEL1 Protein

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SDS-Page: Recombinant Human GRPEL1 Protein [NBP1-49431] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human GRPEL1 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-217 of Human GRPEL1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCTATKQKNS GQNLEEDMGQ SEQKADPPAT EKTLLEEKVK LEEQLKETVE KYKRALADTE NLRQRSQKLV EEAKLYGIQA FCKDLLEVAD VLEKATQCVP KEEIKDDNPH LKNLYEGLVM TEVQIQKVFT KHGLLKLNPV GAKFDPYEHE ALFHTPVEGK EPGTVALVSK VGYKLHGRTL RPALVGVVKE A

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GRPEL1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
23.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human GRPEL1 Protein

  • FLJ25609
  • GREPEL1
  • grpE protein homolog 1, mitochondrial
  • GrpE-like 1, mitochondrial (E. coli)
  • GrpE-like protein cochaperone
  • HMGEmt-GrpE#1
  • Mt-GrpE#1

Background

GRPEL1 is an essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. This protein seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Recombinant human GRPEL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GRPEL1