GRK3 Antibody (SR2232) - BSA Free

Western Blot: GRK3 Antibody (SR2232) [NBP3-21869] - Western blot analysis using NBP3-21869 in Ramos cell lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: SR2232
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GRK3 Antibody (SR2232) - BSA Free Summary

• Description: Novus Biologicals Rabbit GRK3 Antibody (SR2232) - BSA Free (NBP3-21869) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human GRK3 (Uniprot #: P35626)
• Specificity: Detects endogenous levels of total GRK3
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GRK3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GRK3 Antibody (SR2232) - BSA Free

• ADRBK2
• adrenergic, beta, receptor kinase 2
• BARK2
• BARK2beta-adrenergic receptor kinase 2
• Beta-ARK-2
• EC 2.7.11
• EC 2.7.11.15
• G-protein-coupled receptor kinase 3
• GRK3
• GRK3Beta-ARK-2

Background

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second essenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1; two forms of beta-adrenergic receptor kinase, GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2); IT-11 (GRK 4); GRK 5, GRK 6 and GRK 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRK3