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Granzyme M Overexpression Lysate

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Western Blot: Granzyme M Overexpression Lysate (Adult Normal) [NBL1-11420] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Granzyme M.
Western Blot: Granzyme M Overexpression Lysate (Adult Normal) [NBL1-11420] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Granzyme M.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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Granzyme M Overexpression Lysate Summary

Description

Granzyme M Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC206497

Accession#: NM_005317

Protein Tag: C-MYC/DDK
Gene
GZMM

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Granzyme M Overexpression Lysate

  • EC 3.4.21
  • EC 3.4.21.-
  • granzyme M (lymphocyte met-ase 1)
  • granzyme M
  • Hu-Met-1
  • LMET1
  • lymphocyte met-ase 1
  • Met-1 serine protease
  • MET1met-ase
  • Met-ase
  • Natural killer cell granular protease

Background

Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: granzyme A, granzyme B, granzyme H, and Met-ase, also known as granzyme M. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Immunocytochemistry/Immunofluorescence: SerpinB9 Antibody [NBP2-93879] - Analysis of HeLa cells using SerpinB9 at dilution of 1:100. Blue: DAPI for nuclear staining.
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Bioinformatics

Gene Symbol GZMM